📖Complete GuideUpdated 2026🔬Evidence-Based

Peptide Reconstitution Guide

How to Mix & Prep Safely

Step-by-step guide on peptide reconstitution. Learn how to use bacteriostatic water, calculate ratios, and handle vials safely. Guide by Shotlee.

Dashboard
Your complete health overview in one place
🔥
14-day streak
Logged 18 of 18 scheduled shots
Next Shot Reminder
ACTIVE MEDICATIONS
Peptide ReconstitutionB12
PEPTIDE RECONSTITUTION
🔥 14
2
days away
Thursday · 7.5mg
Medication Supply
Peptide Reconstitution
6.2 / 10 mg · Vial
~42 days left · Mar 15
B12
4.4 / 5 ml · Vial
~88 days left · May 8
Retatrutide
1.2 / 5 mg · Vial
~6 days left · refill soon
Medication Levels
7 Days2 Weeks1 Month90 Days
CURRENT LEVEL
5.42mg
Mar 6
Today
ACTIVE MEDICATIONS
Peptide ReconstitutionRetatrutide
0mg2.7mg5.4mg
18
Total Injections
💉 All time
122/78
Average BP
🩺 mmHg
8.4
Avg Mood
😊 /10
Health Chart
1mAll
218.6lb
↓ 8.4 lb · 3.7%
WeightInjectionsBPMood
2282222162102277.5mg7.5mg4mg10mg218
Feb 6Feb 14Feb 22Mar 2Today
Photos
12 photos · 2w streak
Week 1
Week 7
Today
Scheduled Reminders
Weight
Due today
Mood
Done
Blood Pressure
Tomorrow
Body Measurements
In 3 days
Dashboard
Your complete health overview in one place
🔥
14-day streak
Logged 18 of 18 scheduled shots
Next Shot Reminder
ACTIVE MEDICATIONS
Peptide ReconstitutionB12
PEPTIDE RECONSTITUTION
🔥 14
2
days away
Thursday · 7.5mg
Medication Supply
Peptide Reconstitution
6.2 / 10 mg · Vial
~42 days left · Mar 15
B12
4.4 / 5 ml · Vial
~88 days left · May 8
Retatrutide
1.2 / 5 mg · Vial
~6 days left · refill soon
Medication Levels
7 Days2 Weeks1 Month90 Days
CURRENT LEVEL
5.42mg
Mar 6
Today
ACTIVE MEDICATIONS
Peptide ReconstitutionRetatrutide
0mg2.7mg5.4mg
18
Total Injections
💉 All time
122/78
Average BP
🩺 mmHg
8.4
Avg Mood
😊 /10
Health Chart
1mAll
218.6lb
↓ 8.4 lb · 3.7%
WeightInjectionsBPMood
2282222162102277.5mg7.5mg4mg10mg218
Feb 6Feb 14Feb 22Mar 2Today
Photos
12 photos · 2w streak
Week 1
Week 7
Today
Scheduled Reminders
Weight
Due today
Mood
Done
Blood Pressure
Tomorrow
Body Measurements
In 3 days
01BACKGROUND

Safety, Sterility & Precision

Proper reconstitution is the most critical step in research. Using Bacteriostatic Water correctly and maintaining sterility ensures the integrity of your Tirzepatide, Retatrutide, or other peptides. After reconstitution, use Shotlee to log your vial prep date and track the 28-day use window automatically.

02DEEP DIVE

The 5-Step Prep Process

Sanitize Everything: Use 70% isopropyl alcohol pads on the tops of both the peptide vial and the BAC water vial.

Draw BAC Water: Use a sterile syringe to draw the exact amount of Bacteriostatic Water needed for your ratio.

The Slow Drip: Inject the water into the peptide vial at an angle, letting it run down the glass wall. Never spray directly onto the powder.

Gently Swirl: Do not shake. Gently swirl the vial until the solution is clear. Some peptides (like BPC-157) dissolve instantly; others may take minutes.

Storage: Once mixed, most peptides must be refrigerated to prevent degradation.

03DEEP DIVE

Need a Calculator?

Don't guess your dosage. Use our built-in tools for perfect math.

04FAQ

Guide FAQs

Step-by-step guide on peptide reconstitution. Learn how to use bacteriostatic water, calculate ratios, and handle vials safely. Guide by Shotlee.

Yes. Shotlee supports tracking Peptide Reconstitution doses, side effects, and health metrics. It is free to use.

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📚References & sources

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