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📖Complete Guide✅Updated 2026🔬Evidence-Based

Rapamycin Guide (2026)

Complete Guide & Evidence (2026)

mTOR inhibitor and the most studied longevity drug. FDA-approved immunosuppressant repurposed at low intermittent doses (2-6mg weekly) for anti-aging via.

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Dashboard
Your complete health overview in one place
🔥
14-day streak
Logged 18 of 18 scheduled shots
Next Shot Reminder
⌄
ACTIVE MEDICATIONS
RapamycinB12
RAPAMYCIN
🔥 14
2
days away
Thursday · 7.5mg
⌄
Medication Supply
Rapamycin
6.2 / 10 mg · Vial
~42 days left · Mar 15
B12
4.4 / 5 ml · Vial
~88 days left · May 8
Retatrutide
1.2 / 5 mg · Vial
~6 days left · refill soon
Medication Levels
7 Days2 Weeks1 Month90 Days
CURRENT LEVEL
5.42mg
Mar 6
Today
ACTIVE MEDICATIONS
RapamycinRetatrutide
0mg2.7mg5.4mg
18
Total Injections
💉 All time
122/78
Average BP
🩺 mmHg
8.4
Avg Mood
😊 /10
Health Chart
1mAll
218.6lb
↓ 8.4 lb · 3.7%
WeightInjectionsBPMood
2282222162102277.5mg7.5mg4mg10mg218
Feb 6Feb 14Feb 22Mar 2Today
Photos
12 photos · 2w streak
Week 1
Week 7
Today
Scheduled Reminders
Weight
Due today
Mood
Done
Blood Pressure
Tomorrow
Body Measurements
In 3 days
Dashboard
Your complete health overview in one place
🔥
14-day streak
Logged 18 of 18 scheduled shots
Next Shot Reminder
⌄
ACTIVE MEDICATIONS
RapamycinB12
RAPAMYCIN
🔥 14
2
days away
Thursday · 7.5mg
⌄
Medication Supply
Rapamycin
6.2 / 10 mg · Vial
~42 days left · Mar 15
B12
4.4 / 5 ml · Vial
~88 days left · May 8
Retatrutide
1.2 / 5 mg · Vial
~6 days left · refill soon
Medication Levels
7 Days2 Weeks1 Month90 Days
CURRENT LEVEL
5.42mg
Mar 6
Today
ACTIVE MEDICATIONS
RapamycinRetatrutide
0mg2.7mg5.4mg
18
Total Injections
💉 All time
122/78
Average BP
🩺 mmHg
8.4
Avg Mood
😊 /10
Health Chart
1mAll
218.6lb
↓ 8.4 lb · 3.7%
WeightInjectionsBPMood
2282222162102277.5mg7.5mg4mg10mg218
Feb 6Feb 14Feb 22Mar 2Today
Photos
12 photos · 2w streak
Week 1
Week 7
Today
Scheduled Reminders
Weight
Due today
Mood
Done
Blood Pressure
Tomorrow
Body Measurements
In 3 days
01WHAT TO TRACK

mTOR Inhibition: The Core Mechanism

01
🔬
mTORC1 Inhibition
Blocks nutrient-sensing mTOR complex 1. Triggers autophagy (cellular self-cleaning). Reduces protein synthesis — extends cell lifespan
02
🔬
Caloric Restriction Mimetic
Mimics CR signaling without eating less. Upregulates AMPK-like stress response pathways. Reduces IGF-1 and insulin signaling
03
🔬
Senolytic Properties
Reduces senescent (zombie) cell burden. Slows SASP (senescence-associated secretory phenotype). Synergistic with senolytics like fisetin
02BACKGROUND

Key Research Findings

Median lifespan increase in NIA ITP mouse studies — even when started at ~65 human-equivalent age.

Most common pulsed weekly dose used in longevity protocols with physician oversight.

Time to peak autophagy upregulation after a single rapamycin dose in animal models.

03DEEP DIVE

Pulsed Rapamycin Protocol

Key insight: Pulsed dosing (weekly) achieves mTOR inhibition with far less immunosuppression than daily dosing. GFJ (grapefruit juice) co-ingestion can potentiate absorption 2–3×. Always use under physician supervision with regular bloodwork.

04DEEP DIVE

Rapamycin Research Timeline

FDA approved sirolimus (rapamycin) for kidney transplant rejection prevention.

NIA ITP study: rapamycin extends lifespan in mice even when started late in life.

Human studies in healthy elderly: rapamycin improves immune response to flu vaccine.

PEARL trial (2024–2026) studying rapamycin safety/efficacy for healthy aging in humans.

05WHAT TO TRACK

Vital Protocol FAQs

01
🧬
What is rapamycin and how does it work for longevity?
Rapamycin (sirolimus) is an mTOR (mechanistic target of rapamycin) inhibitor originally developed as an immunosuppressant for organ transplants. For longevity, it works by inhibiting mTORC1, the cellu
02
💉
What is the longevity dose of rapamycin?
The most widely used longevity protocol is pulsed weekly dosing at 5–10 mg once per week (not daily). This protocol — popularized by researchers like Dr. Matt Kaeberlein and Dr. Peter Attia — achieves
03
🔬
Has rapamycin extended lifespan in animals?
Yes — rapamycin is one of the most reproducible lifespan-extending interventions in animals. In the NIA Interventions Testing Program (ITP), rapamycin extended median lifespan in mice by 9–14% even wh
04
⚠️
What are the risks of rapamycin for longevity use?
The primary concerns with rapamycin at longevity doses include: immune suppression (increases infection risk), impaired wound healing, metabolic effects (potential glucose intolerance at higher doses)
06FAQ

Guide FAQs

mTOR inhibitor and the most studied longevity drug. FDA-approved immunosuppressant repurposed at low intermittent doses (2-6mg weekly) for anti-aging via.

Yes. Shotlee supports tracking Rapamycin doses, side effects, and health metrics. It is free to use.

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Track Your Rapamycin Protocol in Shotlee

Free dose logging, side effect tracking, and health metric monitoring for your complete protocol.

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📚References & sources

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