What is NAD+ and Why Track It?

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme found in every living cell. It is essential for energy metabolism, DNA repair, and activation of sirtuins — proteins central to longevity signaling. NAD+ levels decline significantly with age, and this decline is linked to metabolic dysfunction, inflammation, and age-related disease.

Supplements like NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) raise cellular NAD+ by providing metabolic precursors the body uses to synthesize it. Research from Harvard, MIT, and Washington University has demonstrated meaningful NAD+ restoration in animal studies, with human trials actively underway.

Tracking your NAD+ protocol systematically — dose, timing, form, and subjective response — is the best way to optimize your personal longevity stack and notice meaningful changes over time.

Common NAD+ Protocol Forms

NMN
250–1,000mg/day

Most studied oral precursor. Converts directly to NAD+. Take in the morning for circadian alignment.

NR
250–500mg/day

Nicotinamide riboside — slightly different metabolic pathway. Often less expensive than NMN.

IV NAD+
250–1,000mg IV

Direct infusion bypasses conversion steps. Faster effect but requires clinic visits.

FAQ

What is the difference between NAD+, NMN, and NR?

NAD+ is the active coenzyme. NMN and NR are precursors your body converts into NAD+. All three aim to raise cellular NAD+. NMN and NR are taken as oral supplements; IV NAD+ delivers it directly.

What is a typical NMN dose?

Common doses range from 250–1,000mg/day taken in the morning. David Sinclair publicly takes 1,000mg NMN daily, though optimal human doses are still under investigation in clinical trials.

Should I take NMN in the morning or evening?

Most users take NMN in the morning, as NAD+ is involved in energy metabolism and circadian signaling. Evening dosing may interfere with sleep for some people.

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